P-105: Gibberellic Acid Can Decrease In Vitro Fertilization Success Rate in Adult Male Rats

Background: Enzymatic and histological change in the testicular cells of rats treated orally, intraperitoneally and intradermally for 45 days with Gibberellic acid (GBA) in independent studies is reported. This study was conducted to evaluate in vitro fertilization (IVF) rate and preimplantation embryonic development in GBA treated adult male rats. Materials and Methods: Thirty six adult male Wistar rat was divided into four groups as following: 1. control group; 2. control-sham group; 3. Low-dose of GBA and 4. High dose of GBA. All injections were intraperitoneally (IP) and every other day. 0.5 CC 1% methanol, 2 mg/kg and 20 mg/kg body weight GBA was injected to groups 2, 3 and 4, respectively. To perform IVF, oocytes from healthy untreated female rats and sperm from mentioned groupswere collected on days 15, 30 and 45 of treatment (three rats for each day of each group) and mR1ECM medium was used. Results: GBA had significantly (p<0.05) decreased oocyte fertilization rate and blastocyst stage embryos rate in day 45 of low dose and days 30 and 45 of high dose in comparison with groups 1 and 2. Also there was a significant (p<0.05) decrease in two-cell stage embryos rate in groups 3 and 4 on days 30 and 45compared to control and control-sham groups. Conclusion: Intraperitoneal administration of GBA acid may have a deleterious effect on IVF success and preimplantation embryo development which may be dosedependent and time-dependent.